Hotspot

trnN-GUU-ndhF

IRb · 114852-116360 · 1509 bp

Back to Bombycidendron

Pi 0.00738
MarkerSeek score 63.20
Primer available yes
Label trnN-GUU-ndhF

Feature Metadata

label_name
trnN-GUU-ndhF
feature_type
igs
region
IRb
length_bp
1509
variable_sites
11
pi
0.00738
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ndhF_p1 ACGACCAGTCAGTTAACAGC ATATGTAGGGGGAAGTCGCA 50.0 50.0 57.8 57.9 1649-1691 bp 1.0 79.5
trnN-GUU-ndhF_p2 ACGACCAGTCAGTTAACAGC TATGTAGGGGGAAGTCGCAT 50.0 50.0 57.8 57.9 1648-1690 bp 1.0 79.5
trnN-GUU-ndhF_p3 AGGATTCGAACCTACGACCA ATATGTAGGGGGAAGTCGCA 50.0 50.0 58.2 57.9 1662-1704 bp 1.0 79.4
trnN-GUU-ndhF_p4 AGGATTCGAACCTACGACCA TATGTAGGGGGAAGTCGCAT 50.0 50.0 58.2 57.9 1661-1703 bp 1.0 79.4
trnN-GUU-ndhF_p5 GGATTCGAACCTACGACCAG TATGTAGGGGGAAGTCGCAT 55.0 50.0 57.8 57.9 1660-1702 bp 1.0 79.0