Hotspot

petD-rpoA

LSC · 82878-83127 · 250 bp

Back to Bombycidendron

Pi 0.036

MarkerSeek score 73.20

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 250
variable_sites: 9
pi: 0.036
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GATTGGTACTGCAGTAGCCC	TGGACCTTTTGAATAACAGTCGA	55.0	39.1	58.0	58.0	430-433 bp	1.0	57.0
petD-rpoA_p2	AGCCCTTTGGTTGGGTATTG	TGGACCTTTTGAATAACAGTCGA	50.0	39.1	58.1	58.0	415-418 bp	1.0	56.9
petD-rpoA_p3	GGTTGGGTATTGGAGCAACA	TGGACCTTTTGAATAACAGTCGA	50.0	39.1	58.1	58.0	407-410 bp	1.0	56.8
petD-rpoA_p4	TGGTTGGGTATTGGAGCAAC	TGGACCTTTTGAATAACAGTCGA	50.0	39.1	58.1	58.0	408-411 bp	1.0	56.8
petD-rpoA_p5	AGTAGCCCTTTGGTTGGGTA	TGGACCTTTTGAATAACAGTCGA	50.0	39.1	58.2	58.0	418-421 bp	1.0	55.4