Hotspot

rpl20-rps12

LSC · 73876-74629 · 754 bp

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Pi 0.012

MarkerSeek score 62.70

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 754
variable_sites: 9
pi: 0.012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGAGCCCCTCGAAA	CGCCGAGGAACATGTACTAG	50.0	55.0	57.9	57.9	870-874 bp	1.0	79.0
rpl20-rps12_p2	TTGAATGAGCCCCTCGAAAG	CGCCGAGGAACATGTACTAG	50.0	55.0	57.9	57.9	869-873 bp	1.0	79.0
rpl20-rps12_p3	TTCGACGTCTCCGAGCTATA	CGCCGAGGAACATGTACTAG	50.0	55.0	57.8	57.9	824-828 bp	1.0	78.2
rpl20-rps12_p4	TCGACGTCTCCGAGCTATAT	CGCCGAGGAACATGTACTAG	50.0	55.0	57.5	57.9	823-827 bp	1.0	76.4
rpl20-rps12_p5	TTGAATGAGCCCCTCGAAAG	GCCGAGGAACATGTACTAGG	50.0	55.0	57.9	57.5	868-872 bp	1.0	75.9