Hotspot

petA-psbJ

LSC · 67526-68610 · 1085 bp

Back to Bombycidendron

Pi 0.0476

MarkerSeek score 90.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1085
variable_sites: 49
pi: 0.0476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 90.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	ATTCTGGATTGGGTTCGTCC	50.0	50.0	57.8	57.6	1213-1231 bp	1.0	82.2
petA-psbJ_p2	TGAGAAGGTTCAAGTGTCCG	ATTCTGGATTGGGTTCGTCC	50.0	50.0	57.5	57.6	1145-1163 bp	1.0	79.6
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	ATTCTGGATTGGGTTCGTCC	50.0	50.0	58.7	57.6	1220-1238 bp	1.0	78.0
petA-psbJ_p4	GAGAAGGTTCAAGTGTCCGA	ATTCTGGATTGGGTTCGTCC	50.0	50.0	57.2	57.6	1144-1162 bp	1.0	77.3
petA-psbJ_p5	GCCTTTTGTTCTTCTTGGCA	ATTCTGGATTGGGTTCGTCC	45.0	50.0	57.1	57.6	1212-1230 bp	1.0	76.3