Hotspot

atpB-rbcL

LSC · 58247-59393 · 1147 bp

Back to Bombycidendron

Pi 0.0101

MarkerSeek score 64.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 1147
variable_sites: 11
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTCAAGCGTGGAAACTCCA	AACACCGGCTTTGAATCCAA	45.0	45.0	58.0	58.3	1192-1247 bp	1.0	81.9
atpB-rbcL_p2	TTTCAAGCGTGGAAACTCCA	TAACACCGGCTTTGAATCCA	45.0	45.0	58.0	57.4	1193-1248 bp	1.0	79.8
atpB-rbcL_p3	TTTCAAGCGTGGAAACTCCA	GGCTTTGAATCCAACACCTG	45.0	50.0	58.0	57.3	1186-1241 bp	1.0	78.5
atpB-rbcL_p4	TTTCAAGCGTGGAAACTCCA	CGGCTTTGAATCCAACACCT	45.0	50.0	58.0	58.8	1187-1242 bp	1.0	78.3
atpB-rbcL_p5	TTTCAAGCGTGGAAACTCCA	ACCGGCTTTGAATCCAACAC	45.0	50.0	58.0	59.0	1189-1244 bp	1.0	76.0