Hotspot

rps16-trnQ-UUG

LSC · 6678-7264 · 587 bp

Back to Bombycidendron

Pi 0.00532
MarkerSeek score 65.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
587
variable_sites
3
pi
0.00532
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AGTGGTGGGTTTTGACATCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 678-699 bp 1.0 82.4
rps16-trnQ-UUG_p2 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 657-678 bp 1.0 82.3
rps16-trnQ-UUG_p3 GCCGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 655-676 bp 1.0 82.3
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 639-660 bp 1.0 81.5
rps16-trnQ-UUG_p5 TTTTGACATCCACAGCCGAT GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 669-690 bp 1.0 80.9