Hotspot

rpoC1

LSC · 22000-24823 · 2824 bp

Back to Bombax

Pi 0.000354
MarkerSeek score 56.20
Primer available yes
Label rpoC1

Feature Metadata

label_name
rpoC1
feature_type
gene
region
LSC
length_bp
2824
variable_sites
1
pi
0.000354
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1_p1 GGTCCTAGTTGAACCGGAGA TTGCTCGTCCGAGAATTACG 55.0 50.0 58.4 58.1 3000 bp 1.0 75.7
rpoC1_p2 TAGTTGAACCGGAGAGACCC TTGCTCGTCCGAGAATTACG 55.0 50.0 58.4 58.1 2995 bp 1.0 75.7
rpoC1_p3 GGTCCTAGTTGAACCGGAGA ACGATCTTTGGCTCTGGAAC 55.0 50.0 58.4 57.9 2983 bp 1.0 75.7
rpoC1_p4 TAGTTGAACCGGAGAGACCC ACGATCTTTGGCTCTGGAAC 55.0 50.0 58.4 57.9 2978 bp 1.0 75.7
rpoC1_p5 TAGTTGAACCGGAGAGACCC CGATCTTTGGCTCTGGAACT 55.0 50.0 58.4 57.6 2977 bp 1.0 73.3