Hotspot

rbcL

LSC · 59174-60655 · 1482 bp

Back to Bombax

Pi 0
MarkerSeek score 62.30
Primer available yes
Label rbcL

Feature Metadata

label_name
rbcL
feature_type
gene
region
LSC
length_bp
1482
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL_p1 AGACCTTGTTGTTGCGAGAA TGGGCCGAGTTTAATTGCAA 45.0 45.0 57.7 58.4 1526-1580 bp 1.0 77.2
rbcL_p2 AGACCTTGTTGTTGCGAGAA TTGGGCCGAGTTTAATTGCA 45.0 45.0 57.7 58.4 1527-1581 bp 1.0 77.2
rbcL_p3 TGAGTTGTAGGGAGGGACTT TGGGCCGAGTTTAATTGCAA 50.0 45.0 57.3 58.4 1495-1549 bp 1.0 74.1
rbcL_p4 TGAGTTGTAGGGAGGGACTT TTGGGCCGAGTTTAATTGCA 50.0 45.0 57.3 58.4 1496-1550 bp 1.0 74.1
rbcL_p5 AGTAGACCTTGTTGTTGCGA TGGGCCGAGTTTAATTGCAA 45.0 45.0 57.1 58.4 1529-1583 bp 1.0 72.5