Hotspot

petD

LSC · 81422-82679 · 1258 bp

Back to Bombax

Pi 0
MarkerSeek score 61.80
Primer available yes
Label petD

Feature Metadata

label_name
petD
feature_type
gene
region
LSC
length_bp
1258
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD_p1 TTCCCCGAAGAGAAAATGGA ACTCCCTAGATACGCACATCA 45.0 47.6 56.4 58.1 1285-1324 bp 1.0 61.5
petD_p2 TCCCCGAAGAGAAAATGGAT ACTCCCTAGATACGCACATCA 45.0 47.6 56.2 58.1 1284-1323 bp 1.0 59.6
petD_p3 ATTCCCCGAAGAGAAAATGGA ACTCCCTAGATACGCACATCA 42.9 47.6 57.0 58.1 1286-1325 bp 1.0 57.8
petD_p4 TCCCCGAAGAGAAAATGGATT ACTCCCTAGATACGCACATCA 42.9 47.6 57.0 58.1 1284-1323 bp 1.0 57.8
petD_p5 TTCCCCGAAGAGAAAATGGAT ACTCCCTAGATACGCACATCA 42.9 47.6 57.0 58.1 1285-1324 bp 1.0 57.8