Hotspot

clpP

LSC · 74651-76860 · 2210 bp

Back to Bombax

Pi 0
MarkerSeek score 59.40
Primer available yes
Label clpP

Feature Metadata

label_name
clpP
feature_type
gene
region
LSC
length_bp
2210
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP_p1 TGGATTGGTGTTAAATCGTTGC ACGCTTCCACATCAAAGTGA 40.9 45.0 57.8 57.7 2276-2296 bp 1.0 62.3
clpP_p2 GGATTGGTGTTAAATCGTTGCT ACGCTTCCACATCAAAGTGA 40.9 45.0 57.6 57.7 2275-2295 bp 1.0 60.2
clpP_p3 GGATTGGTGTTAAATCGTTGC ACGCTTCCACATCAAAGTGA 42.9 45.0 56.1 57.7 2275-2295 bp 1.0 56.7
clpP_p4 GGTGTTAAATCGTTGCTATTGCT ACGCTTCCACATCAAAGTGA 39.1 45.0 58.0 57.7 2270-2290 bp 1.0 55.5
clpP_p5 TGTGGATTGGTGTTAAATCGTTG ACGCTTCCACATCAAAGTGA 39.1 45.0 57.9 57.7 2278-2298 bp 1.0 54.7