Hotspot

rpl33-rps18

LSC · 72346-72719 · 374 bp

Back to Bombax

Pi 0.0223
MarkerSeek score 68.20
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
374
variable_sites
7
pi
0.0223
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.84
markerseek_score
68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 CGACACAATACGCCTAGTCG TCAATTCGATCCCCCGATTG 55.0 50.0 58.5 57.7 487-547 bp 1.0 78.8
rpl33-rps18_p2 GACACAATACGCCTAGTCGA TCAATTCGATCCCCCGATTG 50.0 50.0 57.2 57.7 486-546 bp 1.0 76.3
rpl33-rps18_p3 TCGACACAATACGCCTAGTC TCAATTCGATCCCCCGATTG 50.0 50.0 57.2 57.7 488-548 bp 1.0 76.3
rpl33-rps18_p4 CGACACAATACGCCTAGTCG ATTCGATCCCCCGATTGGAT 55.0 50.0 58.5 58.6 484-544 bp 1.0 75.7
rpl33-rps18_p5 ACGCCTAGTCGATTGGAATT TCAATTCGATCCCCCGATTG 45.0 50.0 57.0 57.7 478-538 bp 1.0 75.2