Hotspot

petA-psbJ

LSC · 67540-68620 · 1081 bp

Back to Bombax

Pi 0.000925
MarkerSeek score 59.30
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1081
variable_sites
1
pi
0.000925
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CTACGTGTCCAAGGCCTTTT ATTCTGGATTGGGTTCGTCC 50.0 50.0 58.1 57.6 1221 bp 1.0 75.8
petA-psbJ_p2 GGCCTTTTGTTCTTCTTGGC ATTCTGGATTGGGTTCGTCC 50.0 50.0 57.8 57.6 1209 bp 1.0 75.5
petA-psbJ_p3 GATCCACTACGTGTCCAAGG ATTCTGGATTGGGTTCGTCC 55.0 50.0 57.7 57.6 1227 bp 1.0 74.3
petA-psbJ_p4 TACGTGTCCAAGGCCTTTTG ATTCTGGATTGGGTTCGTCC 50.0 50.0 58.4 57.6 1220 bp 1.0 73.5
petA-psbJ_p5 TGTCCAAGGCCTTTTGTTCT ATTCTGGATTGGGTTCGTCC 45.0 50.0 57.5 57.6 1216 bp 1.0 73.1