Hotspot

psaA-ycf3

LSC · 44499-45414 · 916 bp

Back to Bombax

Pi 0
MarkerSeek score 61.90
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
916
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GCCCATTCCTCGAAAGAAGT TGGTTAAAGATCACGAGGCG 50.0 50.0 57.9 58.0 996-1026 bp 1.0 81.4
psaA-ycf3_p2 GCCCATTCCTCGAAAGAAGT ATCAAGCCGCTGAGTATTGG 50.0 50.0 57.9 58.0 1064-1094 bp 1.0 81.0
psaA-ycf3_p3 GCCCATTCCTCGAAAGAAGT GGAAACAGGCTATAGCGCTT 50.0 50.0 57.9 58.0 1046-1076 bp 1.0 81.0
psaA-ycf3_p4 TTCTTGAGAAATGACCCGGC TGGTTAAAGATCACGAGGCG 50.0 50.0 58.2 58.0 1019-1049 bp 1.0 80.9
psaA-ycf3_p5 GCCCATTCCTCGAAAGAAGT TAAAGATCACGAGGCGCTTC 50.0 50.0 57.9 58.1 992-1022 bp 1.0 80.8