Hotspot

psbA-trnK-UUU

LSC · 1851-2132 · 282 bp

Back to Bombax

Pi 0
MarkerSeek score 25.60
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
LSC
length_bp
282
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
25.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 AACACCAAACCATCCGATGT ACTCCATCCGACTAGTTCCG 45.0 55.0 57.7 58.6 429 bp 1.0 72.8
psbA-trnK-UUU_p2 ACGGTTTTCAGTGCTGGTTA ACTCCATCCGACTAGTTCCG 45.0 55.0 57.7 58.6 405 bp 1.0 72.4
psbA-trnK-UUU_p3 AACACCAAACCATCCGATGT ACTAGTTCCGGGTTCGAGTC 45.0 55.0 57.7 58.8 419 bp 1.0 71.1
psbA-trnK-UUU_p4 AACACCAAACCATCCGATGT GACTAGTTCCGGGTTCGAGT 45.0 55.0 57.7 58.8 420 bp 1.0 71.1
psbA-trnK-UUU_p5 ACGGTTTTCAGTGCTGGTTA ACTAGTTCCGGGTTCGAGTC 45.0 55.0 57.7 58.8 395 bp 1.0 70.7