Hotspot

petA-psbJ

LSC · 62804-63433 · 630 bp

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Pi 0.0238

MarkerSeek score 72.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 630
variable_sites: 15
pi: 0.0238
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	ATGGCTCATATTCCGGGTTG	50.0	50.0	57.8	57.7	767 bp	1.0	78.1
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	ATGGCTCATATTCCGGGTTG	50.0	50.0	58.2	57.7	781 bp	1.0	77.9
petA-psbJ_p3	GACCCATTACGTGTCCAAGG	ATGGCTCATATTCCGGGTTG	55.0	50.0	58.3	57.7	785 bp	1.0	77.2
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	CATATTCCGGGTTGGGTTCA	50.0	50.0	57.8	57.6	761 bp	1.0	76.9
petA-psbJ_p5	CATTACGTGTCCAAGGCCTT	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.2	57.6	775 bp	1.0	76.7