Hotspot

ndhC-trnV-UAC

LSC · 50154-51242 · 1089 bp

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Pi 0.00923
MarkerSeek score 61.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1089
variable_sites
10
pi
0.00923
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGGTGCTAAAACTCCGGAAA AGAAGGTCTACGGTTCGAGT 45.0 50.0 57.4 57.8 1215-1221 bp 1.0 74.3
ndhC-trnV-UAC_p2 TTGGTGCTAAAACTCCGGAA AGAAGGTCTACGGTTCGAGT 45.0 50.0 57.4 57.8 1216-1222 bp 1.0 74.3
ndhC-trnV-UAC_p3 CCGGTCCTTCACTAATTGGT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.2 57.8 1231-1237 bp 1.0 73.2
ndhC-trnV-UAC_p4 ATTGGTGCTAAAACTCCGGA AGAAGGTCTACGGTTCGAGT 45.0 50.0 57.1 57.8 1217-1223 bp 1.0 72.5
ndhC-trnV-UAC_p5 TGGTGCTAAAACTCCGGAAA AGCCCAATGCAACAAAATGC 45.0 45.0 57.4 58.5 1297-1303 bp 1.0 72.1