Hotspot

psaA-ycf3

LSC · 41553-42241 · 689 bp

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Pi 0.00435

MarkerSeek score 61.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 689
variable_sites: 3
pi: 0.00435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	GTTCGATCAAGCTGCTGAGT	50.0	50.0	57.9	58.3	877 bp	1.0	77.3
psaA-ycf3_p2	TTGTTCGTGAAAAATGGCCG	GTTCGATCAAGCTGCTGAGT	45.0	50.0	57.9	58.3	904 bp	1.0	77.0
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	ACGAGACGTTTCGAATTCGA	50.0	45.0	57.9	57.7	792 bp	1.0	77.0
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	TGGTTGAAGATCACGAGACG	50.0	50.0	57.9	57.7	804 bp	1.0	76.8
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	GGTTGAAGATCACGAGACGT	50.0	50.0	57.9	57.7	803 bp	1.0	76.7