Hotspot

rpoB-trnC-GCA

LSC · 27171-28114 · 944 bp

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Pi 0.00425

MarkerSeek score 61.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 944
variable_sites: 4
pi: 0.00425
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGTCAAGCCCTGATTCATGA	TTGTTAATCAGGCGACACCC	50.0	50.0	58.0	58.2	1120-1131 bp	1.0	78.7
rpoB-trnC-GCA_p2	AATTCTTCCGTCAAGCCCTG	TTGTTAATCAGGCGACACCC	50.0	50.0	58.2	58.2	1128-1139 bp	1.0	77.6
rpoB-trnC-GCA_p3	CGTCAAGCCCTGATTCATGA	GCGACACCCAGATTTGAACT	50.0	50.0	58.0	58.5	1109-1120 bp	1.0	76.4
rpoB-trnC-GCA_p4	CGTCAAGCCCTGATTCATGA	TAATCAGGCGACACCCAGAT	50.0	50.0	58.0	58.5	1116-1127 bp	1.0	76.2
rpoB-trnC-GCA_p5	CGTCAAGCCCTGATTCATGA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.0	57.5	1084-1095 bp	1.0	76.0