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atpH-atpI

LSC · 14004-14867 · 864 bp

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Pi 0.00349

MarkerSeek score 61.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 864
variable_sites: 3
pi: 0.00349
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	951-952 bp	1.0	79.2
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	921-922 bp	1.0	78.9
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	1007-1008 bp	1.0	78.6
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	977-978 bp	1.0	78.3
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	952-953 bp	1.0	78.1