Hotspot

rps16-trnQ-UUG

LSC · 5913-7179 · 1267 bp

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Pi 0.00884
MarkerSeek score 65.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1267
variable_sites
11
pi
0.00884
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CGGCAGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1354-1358 bp 1.0 81.1
rps16-trnQ-UUG_p2 AATCCACGGCAGATCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1360-1364 bp 1.0 81.0
rps16-trnQ-UUG_p3 GAATCCACGGCAGATCATGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1361-1365 bp 1.0 81.0
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1336-1340 bp 1.0 80.2
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1347-1351 bp 1.0 79.7