Hotspot

trnN-GUU-rpl32

SSC · 106892-110418 · 3527 bp

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Pi 0.0498
MarkerSeek score 76.10
Primer available yes
Label trnN-GUU-rpl32

Feature Metadata

label_name
trnN-GUU-rpl32
feature_type
igs
region
SSC
length_bp
3527
variable_sites
509
pi
0.0498
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rpl32_p1 CCTCCAGGAAGCGTAGTAGA ATACCCCCAGAGACAACCAA 55.0 50.0 57.9 57.9 834 bp 0.888889 82.7
trnN-GUU-rpl32_p2 GTCGGGAGAAATGAACACGA ATACCCCCAGAGACAACCAA 50.0 50.0 57.9 57.9 715 bp 0.888889 82.5
trnN-GUU-rpl32_p3 GTCGGGAGAAATGAACACGA GAGACAACCAAATTTCGGCG 50.0 50.0 57.9 58.0 706 bp 0.333333 57.8
trnN-GUU-rpl32_p4 AGTCGGGAGAAATGAACACG GAGACAACCAAATTTCGGCG 50.0 50.0 57.9 58.0 707 bp 0.333333 57.7
trnN-GUU-rpl32_p5 CCTCCAGGAAGCGTAGTAGA GAGACAACCAAATTTCGGCG 55.0 50.0 57.9 58.0 825 bp 0.333333 57.5