Hotspot

trnC-GCA-trnG-GCC

LSC · 30625-31127 · 503 bp

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Pi 0.0778
MarkerSeek score 81.20
Primer available yes
Label trnC-GCA-trnG-GCC

Feature Metadata

label_name
trnC-GCA-trnG-GCC
feature_type
igs
region
LSC
length_bp
503
variable_sites
112
pi
0.0778
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-trnG-GCC_p1 CCTACGTCTTACCGCTTGAC GTTCGATTCCCGCTATCCG 55.0 57.9 58.1 58.2 550-598 bp 1.0 81.1
trnC-GCA-trnG-GCC_p2 GTCCTACGTCTTACCGCTTG GTTCGATTCCCGCTATCCG 55.0 57.9 58.1 58.2 552-600 bp 1.0 81.1
trnC-GCA-trnG-GCC_p3 CCTACGTCTTACCGCTTGAC GGGTTCGATTCCCGCTATC 55.0 57.9 58.1 57.8 552-600 bp 1.0 80.9
trnC-GCA-trnG-GCC_p4 CCTACGTCTTACCGCTTGAC GGTTCGATTCCCGCTATCC 55.0 57.9 58.1 57.8 551-599 bp 1.0 80.9
trnC-GCA-trnG-GCC_p5 GTCCTACGTCTTACCGCTTG GGTTCGATTCCCGCTATCC 55.0 57.9 58.1 57.8 553-601 bp 1.0 80.9