Hotspot

psbM-trnC-GCA

LSC · 28008-30552 · 2545 bp

Back to Bolbitis

Pi 0.0796
MarkerSeek score 80.70
Primer available yes
Label psbM-trnC-GCA

Feature Metadata

label_name
psbM-trnC-GCA
feature_type
igs
region
LSC
length_bp
2545
variable_sites
561
pi
0.0796
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnC-GCA_p1 GATGAAGATTCGGTCGACCC ACGTGGACACCATACCTGTA 55.0 50.0 58.1 58.1 2457-2959 bp 0.555556 72.5
psbM-trnC-GCA_p2 GATGAAGATTCGGTCGACCC CCGGATCGACGTTGTTTACT 55.0 50.0 58.1 58.0 2383-2885 bp 0.333333 61.3
psbM-trnC-GCA_p3 GGATGAAGATTCGGTCGACC CCGGATCGACGTTGTTTACT 55.0 50.0 58.1 58.0 2384-2886 bp 0.333333 61.3
psbM-trnC-GCA_p4 GATGAAGATTCGGTCGACCC TCCGGATCGACGTTGTTTAC 55.0 50.0 58.1 58.0 2384-2886 bp 0.333333 61.2
psbM-trnC-GCA_p5 GGATGAAGATTCGGTCGACC TCCGGATCGACGTTGTTTAC 55.0 50.0 58.1 58.0 2385-2887 bp 0.333333 61.2