Hotspot

rpoC2-trnD-GUC

LSC · 19971-27140 · 7170 bp

Back to Bolbitis

Pi 0.0278
MarkerSeek score 64.20
Primer available yes
Label rpoC2-trnD-GUC

Feature Metadata

label_name
rpoC2-trnD-GUC
feature_type
igs
region
LSC
length_bp
7170
variable_sites
585
pi
0.0278
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2-trnD-GUC_p1 ATCCGAAGTTGCAGTGCTAG ATTGCTATCGATGGGAGGGA 50.0 50.0 58.0 58.0 951 bp 0.111111 44.1
rpoC2-trnD-GUC_p2 ATCCGAAGTTGCAGTGCTAG TTGCTATCGATGGGAGGGAT 50.0 50.0 58.0 58.0 950 bp 0.111111 44.1
rpoC2-trnD-GUC_p3 GTGATGCATTGATACGCAGC ATTGCTATCGATGGGAGGGA 50.0 50.0 58.0 58.0 716 bp 0.111111 44.0
rpoC2-trnD-GUC_p4 GTGATGCATTGATACGCAGC TTGCTATCGATGGGAGGGAT 50.0 50.0 58.0 58.0 715 bp 0.111111 44.0
rpoC2-trnD-GUC_p5 TGCATTGATACGCAGCATCT TTGCTATCGATGGGAGGGAT 45.0 50.0 58.0 58.0 711 bp 0.111111 43.9