Hotspot

trnS-GCU-ycf12

LSC · 7853-8389 · 537 bp

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Pi 0.0733

MarkerSeek score 78.90

Primer available yes

Label trnS-GCU-ycf12

Feature Metadata

label_name: trnS-GCU-ycf12
feature_type: igs
region: LSC
length_bp: 537
variable_sites: 115
pi: 0.0733
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-ycf12_p1	CAATCCGACGCTTTAGACCA	CCCACCCACTCCTCGTATAT	50.0	55.0	58.0	58.0	762-797 bp	0.666667	77.6
trnS-GCU-ycf12_p2	AATCCGACGCTTTAGACCAC	CCCACCCACTCCTCGTATAT	50.0	55.0	58.0	58.0	761-796 bp	0.666667	77.6
trnS-GCU-ycf12_p3	CGAGGGTCGCTACAATTCAA	CCCACCCACTCCTCGTATAT	50.0	55.0	58.0	58.0	1303-1348 bp	0.666667	77.2
trnS-GCU-ycf12_p4	CAATCCGACGCTTTAGACCA	TCTTTTTGGGATCGTGCCAA	50.0	45.0	58.0	58.0	1098 bp	0.111111	44.3
trnS-GCU-ycf12_p5	CGAGGGTCGCTACAATTCAA	TCTTTTTGGGATCGTGCCAA	50.0	45.0	58.0	58.0	1649 bp	0.111111	44.3