Hotspot

matK-rps16

LSC · 2460-3607 · 1148 bp

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Pi 0.0567

MarkerSeek score 81.70

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 1148
variable_sites: 187
pi: 0.0567
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	CGCGGAATTTCCCTTTTTCC	CGAACGAGGGGATTTTCGAT	50.0	50.0	58.0	58.1	2354-2376 bp	0.777778	80.3
matK-rps16_p2	GCACAAACTTCGAATGAGGC	ACTTGCTATTGCTGCCCTAC	50.0	50.0	58.0	58.0	1602-1609 bp	0.333333	57.6
matK-rps16_p3	CGCGGAATTTCCCTTTTTCC	ACTTGCTATTGCTGCCCTAC	50.0	50.0	58.0	58.0	1927-1934 bp	0.333333	57.2
matK-rps16_p4	CGCGGAATTTCCCTTTTTCC	TTCCTACAACCCCAGGAGAG	50.0	55.0	58.0	58.0	1966 bp	0.111111	44.0
matK-rps16_p5	GCACAAACTTCGAATGAGGC	TTCCTACAACCCCAGGAGAG	50.0	55.0	58.0	58.0	1641 bp	0.111111	43.8