Hotspot

petB

LSC · 78823-80283 · 1461 bp

Back to Boesenbergia

Pi 0.0132

MarkerSeek score 57.40

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1461
variable_sites: 19
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GAATATGAGTGTGTGACTTGT	TCTATCGTTCCTCGCCAGTA	38.1	50.0	53.6	57.7	1575-1584 bp	1.0	42.1
petB_p2	GAATATGAGTGTGTGACTTGTT	TCTATCGTTCCTCGCCAGTA	36.4	50.0	54.4	57.7	1575-1584 bp	1.0	42.1
petB_p3	GAATATGAGTGTGTGACTTGT	ATTGTCTATCGTTCCTCGCC	38.1	50.0	53.6	57.5	1579-1588 bp	1.0	42.1
petB_p4	GAATATGAGTGTGTGACTTGT	ATCGTTCCTCGCCAGTATGA	38.1	50.0	53.6	58.6	1572-1581 bp	1.0	42.1
petB_p5	GAATATGAGTGTGTGACTTGT	TCGTTCCTCGCCAGTATGAT	38.1	50.0	53.6	58.6	1571-1580 bp	1.0	42.1