Hotspot

ndhG-ndhI

SSC · 126681-127082 · 402 bp

Back to Boesenbergia

Pi 0.0262

MarkerSeek score 59.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 402
variable_sites: 10
pi: 0.0262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ACTCCATCTCCAACGGTACA	GTTTCCAATGGTGACTGGGT	50.0	50.0	58.1	58.0	1239-1251 bp	1.0	81.8
ndhG-ndhI_p2	ACTCCATCTCCAACGGTACA	TGTTTCCAATGGTGACTGGG	50.0	50.0	58.1	58.0	1240-1252 bp	1.0	81.8
ndhG-ndhI_p3	AAGAACCAACCCCAACGAAA	GTTTCCAATGGTGACTGGGT	45.0	50.0	57.9	58.0	1072-1084 bp	1.0	81.5
ndhG-ndhI_p4	AGAACCAACCCCAACGAAAA	GTTTCCAATGGTGACTGGGT	45.0	50.0	57.9	58.0	1071-1083 bp	1.0	81.5
ndhG-ndhI_p5	ACTCCATCTCCAACGGTACA	CAGGATGAGAATGGACCAGC	50.0	55.0	58.1	58.0	1440 bp	0.5	59.2