Hotspot

psaC-ndhE

SSC · 125003-125646 · 644 bp

Back to Boesenbergia

Pi 0.0316

MarkerSeek score 68.60

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 644
variable_sites: 20
pi: 0.0316
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GGCAAGCTCGTACACATTGA	AACGCGTATCAACCAATCGA	50.0	45.0	58.6	58.0	745-1007 bp	1.0	78.3
psaC-ndhE_p2	GGCAAGCTCGTACACATTGA	ACGCGTATCAACCAATCGAA	50.0	45.0	58.6	58.0	744-1006 bp	1.0	78.3
psaC-ndhE_p3	GGCAAGCTCGTACACATTGA	CAACGCGTATCAACCAATCG	50.0	50.0	58.6	57.9	746-1008 bp	1.0	77.8
psaC-ndhE_p4	GCAAGCTCGTACACATTGAG	AACGCGTATCAACCAATCGA	50.0	45.0	57.2	58.0	744-1006 bp	1.0	76.5
psaC-ndhE_p5	GCAAGCTCGTACACATTGAG	ACGCGTATCAACCAATCGAA	50.0	45.0	57.2	58.0	743-1005 bp	1.0	76.5