Hotspot

trnD-GUC-trnY-GUA

LSC · 32734-33188 · 455 bp

Back to Boesenbergia

Pi 0.0288

MarkerSeek score 65.70

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 455
variable_sites: 13
pi: 0.0288
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GGATCCTAGTTCGGGACTGA	AATTCTTCCTGGGTCGATGC	55.0	50.0	57.9	58.0	630-634 bp	1.0	81.2
trnD-GUC-trnY-GUA_p2	GATCGGATCCTAGTTCGGGA	AATTCTTCCTGGGTCGATGC	55.0	50.0	58.1	58.0	634-638 bp	1.0	81.0
trnD-GUC-trnY-GUA_p3	GGATCCTAGTTCGGGACTGA	GGGGACGGACTGTAAATTCG	55.0	55.0	57.9	58.4	597-601 bp	1.0	78.9
trnD-GUC-trnY-GUA_p4	ATCGGATCCTAGTTCGGGAC	AATTCTTCCTGGGTCGATGC	55.0	50.0	58.4	58.0	633-637 bp	1.0	78.7
trnD-GUC-trnY-GUA_p5	GGATCCTAGTTCGGGACTGA	GGGACGGACTGTAAATTCGT	55.0	50.0	57.9	57.6	596-600 bp	1.0	78.7