Hotspot

petN-psbM

LSC · 30189-31487 · 1299 bp

Back to Boesenbergia

Pi 0.0195

MarkerSeek score 56.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1299
variable_sites: 25
pi: 0.0195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GTCTACTGACTCCATCCGGA	GCCTTCCAGTCCCAATTGAA	55.0	50.0	57.9	58.1	2506 bp	0.5	59.0
petN-psbM_p2	TATCCAGACTCACGAGGGTC	GCCTTCCAGTCCCAATTGAA	55.0	50.0	57.9	58.1	2422 bp	0.5	59.0
petN-psbM_p3	AGTATGGGGAAGGAGTGGAC	GCCTTCCAGTCCCAATTGAA	55.0	50.0	58.1	58.1	1969 bp	0.5	58.4
petN-psbM_p4	GTATGGGGAAGGAGTGGACT	GCCTTCCAGTCCCAATTGAA	55.0	50.0	58.1	58.1	1968 bp	0.5	58.4
petN-psbM_p5	TATCCAGACTCACGAGGGTC	ATGCCTTCCAGTCCCAATTG	55.0	50.0	57.9	58.1	2424 bp	0.5	58.4