Hotspot

trnS-GCU-trnG-GCC

LSC · 8741-9445 · 705 bp

Back to Boesenbergia

Pi 0.0158

MarkerSeek score 65.10

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 705
variable_sites: 11
pi: 0.0158
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	CGGAGATACCCCTTCCCTTA	50.0	55.0	58.0	57.6	815-818 bp	1.0	78.9
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	CGGAGATACCCCTTCCCTTA	50.0	55.0	58.0	57.6	814-817 bp	1.0	78.9
trnS-GCU-trnG-GCC_p3	AGTATCAAGCGGGTCGAGTA	CGGAGATACCCCTTCCCTTA	50.0	55.0	58.0	57.6	899-902 bp	1.0	78.3
trnS-GCU-trnG-GCC_p4	GAGCGGAGAAAGAGGGATTC	CGGAGATACCCCTTCCCTTA	55.0	55.0	57.8	57.6	871-874 bp	1.0	76.9
trnS-GCU-trnG-GCC_p5	GTCGAGTAATCAGAGCGAGC	CGGAGATACCCCTTCCCTTA	55.0	55.0	58.3	57.6	887-890 bp	1.0	76.7