Hotspot

ycf1

SSC · 124225-127734 · 3510 bp

Pi 0.457

MarkerSeek score 78.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 3510
variable_sites: 1450
pi: 0.457
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGAATTTTCAATCGTGGATAC	AAACGACGGTGGACAATTGA	38.1	45.0	53.4	58.0	501 bp	0.5	20.0
ycf1_p2	CGAATTTTCAATCGTGGATAC	CCATTCGTTTGCCTGGAAAC	38.1	50.0	53.4	57.9	517 bp	0.5	20.0
ycf1_p3	CGAATTTTCAATCGTGGATAC	AAAAAGAACCCACAGGCCAA	38.1	45.0	53.4	58.1	883 bp	0.5	20.0
ycf1_p4	CGAATTTTCAATCGTGGATAC	AACGACGGTGGACAATTGAT	38.1	45.0	53.4	57.8	500 bp	0.5	20.0
ycf1_p5	CGAATTTTCAATCGTGGATAC	ACGACGGTGGACAATTGATT	38.1	45.0	53.4	57.8	499 bp	0.5	20.0