Hotspot

ndhA

SSC · 120177-122315 · 2139 bp

Pi 0.477

MarkerSeek score 79.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2139
variable_sites: 851
pi: 0.477
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CGCGTGAGACAAGGTAATCA	ACCAATGCTACGAGCTTCTG	50.0	50.0	58.0	58.0	2832 bp	0.5	59.8
ndhA_p2	CGCGTGAGACAAGGTAATCA	CAGGCTCTGGAAGGAATTCC	50.0	55.0	58.0	58.0	2666 bp	0.5	59.5
ndhA_p3	CGCGTGAGACAAGGTAATCA	CCGGGGTTGCTGAATATCAA	50.0	50.0	58.0	57.9	2944 bp	0.5	59.5
ndhA_p4	CGCGTGAGACAAGGTAATCA	AACCGGGGTTGCTGAATATC	50.0	50.0	58.0	57.9	2946 bp	0.5	59.5
ndhA_p5	CGCGTGAGACAAGGTAATCA	TTTTAACCGGGGTTGCTGAA	50.0	45.0	58.0	57.9	2950 bp	0.5	59.4