Hotspot

rps15-ycf1

SSC · 123869-124224 · 356 bp

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Pi 0.473
MarkerSeek score 76.10
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
356
variable_sites
164
pi
0.473
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCCCAACGCGTTACATAAGG AAACGACGGTGGACAATTGA 50.0 45.0 58.0 58.0 1446 bp 0.5 59.5
rps15-ycf1_p2 TCCCAACGCGTTACATAAGG CCATTCGTTTGCCTGGAAAC 50.0 50.0 58.0 57.9 1462 bp 0.5 59.4
rps15-ycf1_p3 AACACCATGCATTGATGGGT AAACGACGGTGGACAATTGA 45.0 45.0 58.1 58.0 1309 bp 0.5 59.2
rps15-ycf1_p4 AACACCATGCATTGATGGGT CCATTCGTTTGCCTGGAAAC 45.0 50.0 58.1 57.9 1325 bp 0.5 59.0
rps15-ycf1_p5 AGCTGTTCCGGTCCATTTAC AAACGACGGTGGACAATTGA 50.0 45.0 57.9 58.0 1500 bp 0.5 58.8