Hotspot

psaC-ndhE

SSC · 117955-118214 · 260 bp

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Pi 0.518
MarkerSeek score 76.60
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
260
variable_sites
129
pi
0.518
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 TTTGCCCACTGTACATTGCT TACGTCGTGGTATGGGATCA 45.0 50.0 58.0 57.9 1681 bp 0.5 59.5
psaC-ndhE_p2 AACCAACACAGTCTTCGGTC TACGTCGTGGTATGGGATCA 50.0 50.0 58.1 57.9 1096 bp 0.5 59.2
psaC-ndhE_p3 ACACTCAAGAAATCGGTCGG TACGTCGTGGTATGGGATCA 50.0 50.0 57.9 57.9 1139 bp 0.5 58.9
psaC-ndhE_p4 ACATCCGTCCCAAGGTATCA TACGTCGTGGTATGGGATCA 50.0 50.0 58.1 57.9 1047 bp 0.5 58.5
psaC-ndhE_p5 ACGCTCGAACACATTGTGTA TACGTCGTGGTATGGGATCA 45.0 50.0 57.9 57.9 1006 bp 0.5 58.4