Hotspot

ndhF-trnL-UAG

SSC · 113144-114720 · 1577 bp

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Pi 0.42
MarkerSeek score 76.60
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
1577
variable_sites
469
pi
0.42
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.71
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 CAAGAGGTCGTGTGAACCAA GAAGTAGCAAAGCCTTGGGT 50.0 50.0 58.1 58.1 2598 bp 0.5 58.9
ndhF-trnL-UAG_p2 CAAGAGGTCGTGTGAACCAA TGAAGTAGCAAAGCCTTGGG 50.0 50.0 58.1 58.1 2599 bp 0.5 58.9
ndhF-trnL-UAG_p3 CAAGAGGTCGTGTGAACCAA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.1 57.9 2127 bp 0.5 58.6
ndhF-trnL-UAG_p4 CAAGAGGTCGTGTGAACCAA AGCAGCGTGTCTACCAATTT 50.0 45.0 58.1 57.8 2120 bp 0.5 58.1
ndhF-trnL-UAG_p5 CAAGAGGTCGTGTGAACCAA CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.1 57.8 2125 bp 0.5 58.0