Hotspot

ycf1

SSC · 125984-130108 · 4125 bp

Back to Boehmeria

Pi 0.0183

MarkerSeek score 58.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4125
variable_sites: 259
pi: 0.0183
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTTCGGACCATTCGGGAAAT	TGGAAGACAAAAGTTGCATACA	50.0	36.4	58.0	56.5	1005-1050 bp	1.0	60.3
ycf1_p2	ACGCCGTTATAGGTGCATTT	TGGAAGACAAAAGTTGCATACA	45.0	36.4	58.0	56.5	1057-1102 bp	1.0	60.0
ycf1_p3	AACGCCGTTATAGGTGCATT	TGGAAGACAAAAGTTGCATACA	45.0	36.4	58.0	56.5	1058-1103 bp	1.0	59.9
ycf1_p4	AGATTTGTGCCAAGGCTTCA	TGGAAGACAAAAGTTGCATACA	45.0	36.4	58.0	56.5	1154-1199 bp	1.0	59.7
ycf1_p5	ATTTGTGCCAAGGCTTCAGA	TGGAAGACAAAAGTTGCATACA	45.0	36.4	58.0	56.5	1152-1197 bp	1.0	59.7