Hotspot

rps8

IRa · 159870-160274 · 405 bp

Back to Boehmeria

Pi 0.0214

MarkerSeek score 30.10

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: IRa
length_bp: 405
variable_sites: 13
pi: 0.0214
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 30.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	GAACTCAAACGCACCAATGG	ACGTCTGCCGGTTGAATTAT	50.0	45.0	57.9	57.6	1084-1104 bp	0.25	50.4
rps8_p2	GAACTCAAACGCACCAATGG	ATACGTCTGCCGGTTGAATT	50.0	45.0	57.9	57.6	1086-1106 bp	0.25	50.4
rps8_p3	TTGCCCGGGAATTAAGACAG	ACGTCTGCCGGTTGAATTAT	50.0	45.0	57.9	57.6	981-1001 bp	0.25	50.2
rps8_p4	TTGCCCGGGAATTAAGACAG	ATACGTCTGCCGGTTGAATT	50.0	45.0	57.9	57.6	983-1003 bp	0.25	50.2
rps8_p5	GCCCGGGAATTAAGACAGTT	ACGTCTGCCGGTTGAATTAT	50.0	45.0	57.9	57.6	979-999 bp	0.25	50.2