Hotspot

ccsA-ndhD

SSC · 117335-117623 · 289 bp

Back to Boehmeria

Pi 0.0465

MarkerSeek score 68.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 289
variable_sites: 30
pi: 0.0465
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ACGAAATAACCACGCCAAGT	TGACAAAGTCGAAGCCCTTC	45.0	50.0	58.1	58.1	998-1010 bp	1.0	82.8
ccsA-ndhD_p2	ACGAAATAACCACGCCAAGT	ATGGTAATGCCCCAAACTCC	45.0	50.0	58.1	57.8	1122-1134 bp	1.0	82.4
ccsA-ndhD_p3	ACGAAATAACCACGCCAAGT	GACAAAGTCGAAGCCCTTCT	45.0	50.0	58.1	57.8	997-1009 bp	1.0	82.4
ccsA-ndhD_p4	ACGAAATAACCACGCCAAGT	CTTGGTTGATGATGGTCGGT	45.0	50.0	58.1	57.9	1632-1644 bp	1.0	81.8
ccsA-ndhD_p5	ACGAAATAACCACGCCAAGT	GCGTATGGATTGGTTCGGAT	45.0	50.0	58.1	58.1	1693-1705 bp	0.875	76.4