Hotspot

rpl32-trnL-UAG

SSC · 115435-116222 · 788 bp

Back to Boehmeria

Pi 0.0237
MarkerSeek score 71.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
788
variable_sites
51
pi
0.0237
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCAGCATTGAAAGCCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 817-964 bp 1.0 87.1
rpl32-trnL-UAG_p2 GGCAGCATTGAAAGCCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 818-965 bp 1.0 87.1
rpl32-trnL-UAG_p3 GGCAGCATTGAAAGCCTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.8 57.9 827-974 bp 1.0 86.2
rpl32-trnL-UAG_p4 GGCAGCATTGAAAGCCTTTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.8 57.8 826-973 bp 1.0 85.6
rpl32-trnL-UAG_p5 GGCAGCATTGAAAGCCTTTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.8 57.8 825-972 bp 1.0 85.6