Hotspot

ndhC-trnV-UAC

LSC · 52634-53129 · 496 bp

Back to Boehmeria

Pi 0.0159
MarkerSeek score 61.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
496
variable_sites
28
pi
0.0159
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCCAGAAAATATCATATTCGT GTTCGAGTCCGTATAGCCCT 34.8 55.0 56.1 58.7 524-584 bp 1.0 48.0
ndhC-trnV-UAC_p2 TGCCCAGAAAATATCATATTCGT AGAAGGTCTACGGTTCGAGT 34.8 50.0 56.1 57.8 536-596 bp 1.0 47.9
ndhC-trnV-UAC_p3 TGCCCAGAAAATATCATATTCGT GAAGGTCTACGGTTCGAGTC 34.8 55.0 56.1 57.5 535-595 bp 1.0 47.9
ndhC-trnV-UAC_p4 ATGCCCAGAAAATATCATATTCGT AGAAGGTCTACGGTTCGAGT 33.3 50.0 56.6 57.8 537-597 bp 1.0 47.9
ndhC-trnV-UAC_p5 TGCCCAGAAAATATCATATTCGT AAGGTCTACGGTTCGAGTCC 34.8 55.0 56.1 58.8 534-594 bp 1.0 47.9