Hotspot

psaA-ycf3

LSC · 43483-44261 · 779 bp

Back to Boehmeria

Pi 0.0123

MarkerSeek score 63.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 779
variable_sites: 32
pi: 0.0123
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	884-983 bp	1.0	83.8
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TAGCGCTTACTCCGGGTAAT	50.0	50.0	57.9	58.3	912-1011 bp	1.0	82.0
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TGAAGCGCAGAATTGGTTGA	50.0	45.0	57.9	58.4	887-986 bp	1.0	81.4
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TTGAAGCGCAGAATTGGTTG	50.0	45.0	57.9	57.6	888-987 bp	1.0	81.2
psaA-ycf3_p5	TGTTCTTGAGAAATGGCCCG	AGCGCAGAATTGGTTGAAGA	50.0	45.0	58.5	58.1	909-1008 bp	1.0	80.8