Hotspot

trnK-UUU-rps16

LSC · 5697-6490 · 794 bp

Back to Boehmeria

Pi 0.0227
MarkerSeek score 72.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
794
variable_sites
60
pi
0.0227
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT ATTTCAAAGAAGGCGGGTGT 50.0 45.0 58.2 58.0 865-942 bp 1.0 87.6
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT ATTTCAAAGAAGGCGGGTGT 50.0 45.0 58.2 58.0 864-941 bp 1.0 87.6
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT TCAAAGAAGGCGGGTGTTTT 50.0 45.0 58.2 58.2 861-938 bp 1.0 85.9
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TTCAAAGAAGGCGGGTGTTT 50.0 45.0 58.2 58.2 862-939 bp 1.0 85.9
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TTTCAAAGAAGGCGGGTGTT 50.0 45.0 58.2 58.2 863-940 bp 1.0 85.9