Hotspot

ycf1

SSC · 127474-129720 · 2247 bp

Back to Boechera

Pi 0.0073

MarkerSeek score 59.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 2247
variable_sites: 40
pi: 0.0073
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGCTATCCCAAGTTTCTGCA	TGGAAAGTTGCAAAATCATCA	45.0	33.3	56.8	54.5	2337-2388 bp	1.0	41.6
ycf1_p2	AAGCTATCCCAAGTTTCTGCA	TGGAAAGTTGCAAAATCATCA	42.9	33.3	57.6	54.5	2338-2389 bp	1.0	41.6
ycf1_p3	AGCTATCCCAAGTTTCTGCAA	TGGAAAGTTGCAAAATCATCA	42.9	33.3	57.6	54.5	2337-2388 bp	1.0	41.6
ycf1_p4	AGCTATCCCAAGTTTCTGCA	ATGGAAAGTTGCAAAATCATCA	45.0	31.8	56.8	55.1	2338-2389 bp	1.0	41.6
ycf1_p5	AGCTATCCCAAGTTTCTGCA	TGGAAAGTTGCAAAATCATCAT	45.0	31.8	56.8	55.1	2337-2388 bp	1.0	41.6