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trnP-CCA-psaJ

LSC · 66602-66974 · 373 bp

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Pi 0.0145

MarkerSeek score 57.50

Primer available yes

Label trnP-CCA-psaJ

Feature Metadata

label_name: trnP-CCA-psaJ
feature_type: igs
region: LSC
length_bp: 373
variable_sites: 12
pi: 0.0145
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-CCA-psaJ_p1	ACCCGTGACATTTTGTACCC	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	497-502 bp	1.0	80.0
trnP-CCA-psaJ_p2	CCCGTGACATTTTGTACCCA	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	496-501 bp	1.0	80.0
trnP-CCA-psaJ_p3	ACAGGATTTGAACCCGTGAC	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	508-513 bp	1.0	79.8
trnP-CCA-psaJ_p4	CAGGATTTGAACCCGTGACA	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	507-512 bp	1.0	79.8
trnP-CCA-psaJ_p5	TGACAGGATTTGAACCCGTG	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	510-515 bp	1.0	79.8