Hotspot

accD-psaI

LSC · 58591-59262 · 672 bp

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Pi 0.0151

MarkerSeek score 70.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 672
variable_sites: 24
pi: 0.0151
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TGGATGCAATTGTACCACGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.8	57.9	849-888 bp	1.0	82.3
accD-psaI_p2	TGGATGCAATTGTACCACGT	TGCAATTGCCGGAAAGACTA	45.0	45.0	57.8	57.8	839-878 bp	1.0	81.7
accD-psaI_p3	TGGATGCAATTGTACCACGT	CCGGAAAGACTAGGCCTACT	45.0	55.0	57.8	58.2	831-870 bp	1.0	81.5
accD-psaI_p4	TGGATGCAATTGTACCACGT	CATTGCAATTGCCGGAAAGA	45.0	45.0	57.8	57.6	842-881 bp	1.0	80.3
accD-psaI_p5	TGGATGCAATTGTACCACGT	ATTGCAATTGCCGGAAAGAC	45.0	45.0	57.8	57.6	841-880 bp	1.0	80.3