Hotspot

ndhC-trnV-GUA

LSC · 50401-51243 · 843 bp

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Pi 0.0142

MarkerSeek score 70.30

Primer available yes

Label ndhC-trnV-GUA

Feature Metadata

label_name: ndhC-trnV-GUA
feature_type: igs
region: LSC
length_bp: 843
variable_sites: 29
pi: 0.0142
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-GUA_p1	TGCCAAAACAGGAATAGCACT	TGGGTCTTTGAAAGAGGTCG	42.9	50.0	58.1	57.5	860-1135 bp	1.0	73.0
ndhC-trnV-GUA_p2	TGCCAAAACAGGAATAGCACT	GTTCGAGTCCGTATAGCCCT	42.9	55.0	58.1	58.7	790-1065 bp	1.0	72.0
ndhC-trnV-GUA_p3	TGCCAAAACAGGAATAGCAC	TGGGTCTTTGAAAGAGGTCG	45.0	50.0	56.6	57.5	860-1135 bp	1.0	70.8
ndhC-trnV-GUA_p4	TGCCAAAACAGGAATAGCACT	GGGTCTTTGAAAGAGGTCGA	42.9	50.0	58.1	57.2	859-1134 bp	1.0	70.7
ndhC-trnV-GUA_p5	TGCCAAAACAGGAATAGCACT	TTCGAGTCCGTATAGCCCTA	42.9	50.0	58.1	57.1	789-1064 bp	1.0	70.0