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trnT-ACC-psbD

LSC · 31522-32754 · 1233 bp

Back to Boechera

Pi 0.00787

MarkerSeek score 57.90

Primer available yes

Label trnT-ACC-psbD

Feature Metadata

label_name: trnT-ACC-psbD
feature_type: igs
region: LSC
length_bp: 1233
variable_sites: 24
pi: 0.00787
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-ACC-psbD_p1	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	55.0	45.0	58.1	58.0	1384-1418 bp	1.0	82.2
trnT-ACC-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1387-1421 bp	1.0	82.0
trnT-ACC-psbD_p3	GGCGTAAGTCATCGGTTCAA	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.3	58.0	1361-1395 bp	1.0	80.5
trnT-ACC-psbD_p4	TGGTAGAGTAACGCCATGGT	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.4	58.0	1383-1417 bp	1.0	79.2
trnT-ACC-psbD_p5	GTGGTAGAGTAACGCCATGG	TCCCTCCGTAACCAGTCATC	55.0	55.0	58.1	58.5	1365-1399 bp	1.0	78.4