Hotspot

rps16-trnQ-CAA

LSC · 6174-6602 · 429 bp

Back to Boechera

Pi 0.00917

MarkerSeek score 56.70

Primer available yes

Label rps16-trnQ-CAA

Feature Metadata

label_name: rps16-trnQ-CAA
feature_type: igs
region: LSC
length_bp: 429
variable_sites: 8
pi: 0.00917
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-CAA_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	492-520 bp	1.0	81.8
rps16-trnQ-CAA_p2	CCTTCAATTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	502-530 bp	1.0	81.0
rps16-trnQ-CAA_p3	CCACAGCAGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	518-546 bp	1.0	79.4
rps16-trnQ-CAA_p4	TCCACAGCAGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	519-547 bp	1.0	79.3
rps16-trnQ-CAA_p5	ATCCACAGCAGATCATGTCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.4	58.1	520-548 bp	1.0	77.6